HOW-TO5 min read·March 2025

Peptide Reconstitution Calculator: The Complete Guide

Step-by-step guide to reconstituting lyophilized peptides with BAC water. Includes syringe unit calculations and storage protocols.

Why Reconstitution Matters Most research peptides arrive lyophilized — freeze-dried into a powder. Before injection, they must be dissolved in bacteriostatic water (BAC water). Get this calculation wrong and you'll under-dose or overdose every injection. The MyProtocolStack reconstitution calculator handles this automatically, but understanding the math makes you a better, safer user.

The Core Formula

Concentration = Peptide Amount (mcg) ÷ BAC Water Added (mL)

Dose volume (mL) = Target Dose (mcg) ÷ Concentration (mcg/mL)

Insulin syringe units = Dose volume (mL) × 100

Common Reconstitution Examples

### BPC-157 — 5mg Vial

Add 2 mL BAC water → **2,500 mcg/mL concentration**

·250 mcg dose = 0.1 mL = **10 units** on insulin syringe

·500 mcg dose = 0.2 mL = **20 units** on insulin syringe

### Tesamorelin — 2mg Vial

Add 1 mL BAC water → **2,000 mcg/mL concentration**

·1 mg (1,000 mcg) dose = 0.5 mL = **50 units** on insulin syringe

·2 mg dose = 1 mL = **100 units** on insulin syringe

### GHK-Cu — 50mg Vial

Add 5 mL BAC water → **10,000 mcg/mL concentration**

·1 mg dose = 0.1 mL = **10 units** on insulin syringe

·2 mg dose = 0.2 mL = **20 units** on insulin syringe

### Ipamorelin — 2mg Vial

Add 2 mL BAC water → **1,000 mcg/mL concentration**

·200 mcg dose = 0.2 mL = **20 units** on insulin syringe

·300 mcg dose = 0.3 mL = **30 units** on insulin syringe

BAC Water vs Sterile Water

Always use **bacteriostatic water** (BAC water) — not sterile water, not saline. BAC water contains 0.9% benzyl alcohol which prevents microbial growth and extends reconstituted peptide shelf life to 30–60 days under refrigeration. Sterile water is single-use only and requires you to reconstitute fresh for each injection.

Storage After Reconstitution

·**Refrigerate at 2–8°C (35–46°F)** — the standard fridge shelf

·**Never freeze** after reconstitution — ice crystal formation degrades the peptide

·**Protect from light** — amber vials or keep in a dark drawer

·**Label with date** — most reconstituted peptides are stable 30–60 days refrigerated

·**Discard if cloudy, discolored, or particulate** — these indicate degradation or contamination

Using the MyProtocolStack Calculator

Open the Doses tab → Recon Calc. Enter your vial size (mg), BAC water volume (mL), and target dose (mcg). The calculator instantly shows concentration, draw volume in mL, and exact insulin syringe units — no math required.

The calculator also saves your common setups so you can reference your standard protocol without recalculating each time.

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Not medical advice. Always consult a qualified healthcare provider before starting any peptide protocol. Read full disclaimer →